Carbon dioxide incubators are widely used in the research and production of medicine, immunology, genetics, microbiology, agricultural science, and pharmacy. They have become one of the most commonly used routine instruments in the above-mentioned fields, and they are formed by simulating in an incubator. A similar cell/tissue growth environment in the organism such as constant pH (pH: 7.2-7.4), stable temperature (37 ° C), higher relative humidity (95%), stable CO2 level (5%) ), a device for in vitro culture of cells/tissues.

Users around the world have two basic requirements for carbon dioxide incubators. One is to require carbon dioxide incubators to provide the most accurate and stable control of temperature, carbon dioxide concentration and humidity, so that their research work can progress; The incubator can effectively prevent microbial contamination in the incubator and eliminate pollution regularly to protect the research results and prevent sample loss. Therefore, the teacher who purchases the carbon dioxide incubator is most concerned about its high reliability, prevention and control of pollution, and ease of use.

First, temperature control
1. Heating method:
Both air jacketed and water jacketed, both heating systems are accurate and reliable, and they all have their own advantages and disadvantages. Water jacket heating maintains a constant temperature by enclosing an internal tank through a separate water jacket. The advantage is that water is a good thermal insulation material. When a power failure occurs, the water jacket system can Maintaining temperature accuracy and stability in the incubator for a long period of time (maintaining a constant temperature for 3-4 times that of a gas-tight system) is beneficial to the experimental environment is not stable (such as useful electrical limits, or frequent power outages) ) and users who need to maintain stable culture conditions for a long time. The gas-sleeve heating is to directly heat the inner tank through the heaters in the gas jacket of the tank, and is also called direct heating on six sides. Compared with the water jacket type, the gas-sleeve type has the characteristics of quick heating and rapid recovery of the water-type incubator, which is particularly advantageous for short-term cultivation and the need for frequent switching of the door. In addition, the air-sleeve design is simpler for the user than the water jacket type (water jacket type requires watering, emptying and cleaning of the water tank, and often monitoring the operation of the water tank).

2. Temperature control system:
Maintaining a constant temperature in the incubator is an important factor in maintaining healthy cell growth, so an accurate and reliable temperature control system is an integral part of the incubator. In order to make the incubator work more stably, we recommend that users choose a carbon dioxide incubator with independent triple temperature control functions, namely temperature control inside the box, over temperature alarm control and ambient temperature monitoring.

There are currently very few CO2 incubators on the market, and the Heal Force HF90 gas-capped CO2 incubator can do this. The independent temperature control system of the HF90 ensures that the temperature inside the incubator can be accurately stabilized at the user's set value, and the main heating system has two normal/slow heating modes, which are more resistant to high temperature environments. We know The minimum working temperature of the incubator is generally 5 °C above room temperature. If there is no slow heating mode, it is very easy to generate high temperature in the high temperature in summer (such as room temperature 30 °C); the independent over temperature alarm function of HF90 It can quickly and accurately cut the main heating system of the incubator when the temperature in the incubator is higher than the culture temperature by 1 °C, and sound and light alarm; the ambient temperature monitoring of HF90 can automatically adjust the external heating of the incubator according to the change of ambient temperature. The power of the system achieves the purpose of accurately controlling the temperature inside the cabinet.

HF90 air jacket type high temperature moist heat sterilization CO2 incubator front view

3. Temperature uniformity:
The temperature uniformity in the carbon dioxide incubator box is also the main factor that users need to consider. Generally, the uniformity of the incubator equipped with the fan and the air duct in the tank is much better, and the device also contributes to the temperature and CO2 concentration in the tank. Rapid recovery of relative humidity.

Of course, the optimization of the fan/duct is equally important. The HF90 CO2 incubator's uniquely designed large diameter fan and circulation duct ensure uniformity of temperature and CO2 concentration in the tank. Large-diameter fans can generate large air circulation flow at low speed (low wind speed) compared to fans of other brand incubators, reducing wind speed and reducing vibration inside the box while achieving uniformity. Reducing the wind speed and reducing the vibration also greatly improves the success rate of cell culture in the box.

Second, carbon dioxide concentration control
1. Two control systems:
Measurements are made by an infrared sensor (IR) or a thermal conductivity sensor (TCD). Both sensors are accurate, but each has its own advantages and disadvantages. The working principle of the thermal conductivity sensor to monitor the CO2 concentration is based on the continuous measurement of the thermal conductivity of the internal cavity air. The low thermal conductivity of the input CO2 gas will change the thermal conductivity of the air in the cavity, which will directly produce a concentration directly with the CO2. A proportional electrical signal. Infrared sensor (IR) It uses an optical sensor to detect CO2 levels. The IR system includes an infrared emitter and a sensor. When the CO2 in the tank absorbs part of the infrared light emitted by the emitter, the sensor can detect the amount of infrared radiation, and the amount of absorbed infrared light corresponds to the level of CO2 in the tank. Thus, the concentration of CO2 in the tank can be obtained. Since the IR system determines the CO2 concentration in the tank by infrared reduction, and the particulate matter in the box can reflect or partially absorb the infrared rays, so that the IR system is sensitive to the amount of particulate matter in the tank, the IR sensor is applied to the cultivation with the HEPA high efficiency air filter. The box is more suitable, such as the Heal Force HF160W water jacketed incubator is the HEPA filter with high-precision IR sensor configuration; and the HF90 air-cup incubator is a high-precision TCD sensor.

2. CO2 measurement system automatic calibration function:
No matter which kind of CO2 measurement system will drift after using for a period of time, the drift will directly cause the carbon dioxide concentration in the tank to not stabilize at our set value, which will cause the culture to fail, so we strongly recommend that the user purchase the culture here. The incubator with the automatic calibration of the CO2 measuring system must be selected for the tank.

There are currently very few CO2 incubators on the market, and the Heal Force HF90 gas-capped CO2 incubator can do this. The HF90's advanced “AUTO-START” auto start function automatically adjusts the incubator to the operating parameters you set, and keeps the CO2 concentration in the most accurate and stable state, ensuring that the CO2 concentration does not produce positive or negative drift.

3. CO2 concentration uniformity: This point is similar to the temperature uniformity requirement and will not be described here.

Third, the humidity in the relative humidity chamber is a very important but often overlooked factor for the cultivation work. Maintaining sufficient humidity levels and having a fast enough humidity recovery rate (such as after opening and closing the door) will ensure that the culture fails without excessive drying. At present, most carbon dioxide incubators generate moisture through the evaporation of the humidifying disk (the relative humidity level is about 95%, but the humidity recovery rate is very slow after opening the door). We hereby advise users to choose an incubator with a large evaporation area when purchasing a CO2 incubator, because we know that the larger the evaporation area, the easier it is to reach the maximum relative saturation humidity and the shorter the recovery time after opening and closing the door. .
The entire internal bottom of the Heal Force HF90 CO2 incubator is designed as a reservoir-type chassis, which maximizes evaporation area and enhances evaporation. The reservoir-type chassis design allows the incubator to quickly reach saturation humidity. And the humidity recovery speed is much higher than the carbon dioxide incubator that uses the water tray to humidify.

Comparison of humidity recovery time of CO2 incubator

4. Anti-pollution design and disinfection and sterilization system pollution is a major factor leading to cell culture failure. Therefore, manufacturers of carbon dioxide incubators have designed a variety of different devices to reduce and prevent pollution, the main way is Minimize the areas and surfaces where microorganisms can grow, and combine the automatic elimination of pollution devices to effectively prevent pollution. For example, in view of the fact that CO2 incubators are sometimes accompanied by mold growth during use, in order to ensure that the incubator is protected from contamination and to ensure bio-cleanability in the instrument case, various sterilization methods have been introduced, such as UV disinfection. CO2 incubators (such as Heal Force brand HF151UV and HF212UV carbon dioxide incubators); others designed and produced HEPA filters to filter the air in the incubator to remove 99.97% of particles above 0.3 microns (eg Heal Force brand) HF160W carbon dioxide incubator); In addition, an incubator (such as Heal Force brand HF90 and HF240 carbon dioxide incubators) that can achieve high temperature and humidity in the chamber to kill contaminating microorganisms and achieve sterilization and sterilization purposes has been developed. These devices are essential for cell culture, but which cleaning device is chosen? First of all, we are considering the sterilization ability of various methods. The UV disinfection ability is inversely proportional to the square of the distance from the UV lamp. The farther the distance is, the worse the disinfection ability is. Therefore, the UV disinfection method has its limitations. It is difficult to achieve the requirement of thorough sterilization; the HEPA filter is unable to remove viruses and some tiny bacteria due to the influence of the pore size of the filter membrane, and has its limitations; in comparison, high-temperature disinfection is currently a more effective method of disinfection and sterilization. High temperature sterilization is divided into two categories, one is traditional high temperature dry heat disinfection, and the other is advanced high temperature moist heat sterilization.
Next, we will focus on the advantages and disadvantages of high temperature dry heat and high temperature wet heat. High-temperature damp heat is high in the latent heat of the steam, and has a strong penetrating power, which tends to denature or solidify the protein. Therefore, the sterilization efficiency of the method is higher than that of the dry heat sterilization method. There are three reasons for this: 1 The temperature required for protein coagulation is related to its water content. The higher the water content, the lower the temperature required for solidification to occur. The moist heat sterilized bacterial protein absorbs moisture, so it is easy to coagulate in dry hot air at the same temperature. 2 During the moist heat sterilization process, the steam releases a large amount of latent heat, which accelerates the humidity. Thus, moist heat sterilization is lower than the temperature required for dry heat. For example, at the same temperature, the time required for moist heat sterilization is shorter than dry heat. 3 The penetration of damp heat is larger than that of dry heat, so that the deep part can reach the sterilization temperature, so the damp heat is better than the dry heat. Therefore, high temperature sterilization is not a simple look at the disinfection temperature, mainly to see if it is hot and humid. In addition, from the point of view of use, the hot and humid disinfection is generally controlled at 90 ° C to achieve a very thorough disinfection effect, all the accessories in the incubator are not taken out during the entire disinfection process, can be completely disinfected; and dry heat disinfection in order to achieve Good results, the temperature is generally above 100 ° C, at this temperature, the sensor in the sterilization incubator, HEPA filter, etc. should be taken out during the disinfection process, and then the disinfection is finished, then it is troublesome, the attachment is Can not be disinfected at the same time, and increase the probability of secondary pollution, and in addition to reach a high temperature above 100 ° C, the heating wire of the heating system of the incubator must be thickened, which will lead to increased temperature control of the incubator, uniformity change difference. Therefore, we recommend that users choose an incubator with high temperature moist heat sterilization when purchasing a carbon dioxide incubator.
At present, there are very few carbon dioxide incubators with high-temperature moist heat sterilization function on the market, and the Heal Force HF90 gas-capped carbon dioxide incubator can do it. The HF90 incubator contains a 90°C high temperature moist heat sterilization system, combined with its electrolytically polished stainless steel rounded corner inner cavity design, tank condensate control system, CO2 inlet HEPA filtration, external door switch detection system, and three Anti-pollution design such as inner glass door is really an ideal carbon dioxide incubator.

HF90 CO2 incubator with 3 small doors

V. Other factors The volume of the carbon dioxide incubator is also a factor that cannot be ignored. It is not enough to buy small, and it is wasteful and takes up space. The optional volume of the CO2 incubator is very wide and each type has a different volume. At this point, you need to have a more accurate understanding of the range of required incubator volume before purchase, and reserve a little space on this basis to ensure that it is needed.
In addition, each user wants the instrument to be easy to use, micro-processing control system and other various functional accessories (such as high temperature automatic adjustment and alarm device, CO2 alarm device, password protection setting, automatic calibration system, LCD display system). The use of, etc., makes the operation and control of the carbon dioxide incubator very simple. Although different micro-processing systems have different names, their principles and control effects are not different. You don't have to care too much about the difference between their names when purchasing. The key is to make it easy to use and easy to operate.

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